Sample prep

Image
Title
AGM
Description of document #1

AGM TaqMan assay protocol - Part 1 - individual egg masses: This is a step-by-step description of the procedure developed for the analysis of individual gypsy moth egg masses. It can also be used for analysis of other life stages, but the primary focus was egg samples. This suite of assays is not meant to be used for the detection of rare AGM individuals in bulk samples (see Part 2).

Reference publication: Stewart D, Zahiri R, Djoumad A, Freschi L, Lamarche J, Holden D, Cervantes S, Ojeda DI, Potvin A, Nisole A, Béliveau C, Capron A, Kimoto T, Day B, Yueh H, Duff C, Levesque RC, Hamelin RC, Cusson M (2016). A multi-species TaqMan PCR assay for the identification of Asian gypsy moths (Lymantria spp.) and other invasive lymantriines of biosecurity concern to North America. PLoS ONE 11, e0160878.

Description of document #2

AGM TaqMan assay protocol - Part 2 - Bulk pheromone trap samples: This is a step-by-step description of the procedure developed for the detection of rare Asian gypsy moths in bulk pheromone trap samples.

Reference publication: This user manual is based on the following publication: Stewart D, Nisole A, Djoumad A, Zahiri R, Lamarche J, Levesque RC, Hamelin RC, Cusson M. 2018. A needle in a haystack: a multigene TaqMan assay for the detection of Asian gypsy moths in bulk pheromone trap samples. Biological Invasions, accepted for publication.

Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
PR
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
ALB
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
DED
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4

Extractions

Image
Title
DNA
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
RNA
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4

PCR / RT-PCR

Image
Title
AGM
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
PR
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
ALB
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
DED
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4

Sequencing

Image
Title
Library Preparation
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
Sequencing
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4
Image
Title
Data Processing
Document #1
Description of document #1
Document #2
Description of document #2
Document #3
Description of document #3
Document #4
Description of document #4

Other protocols